Abstract Explore the nursing essentials and feeding methods for premature infants and their clinical effects. We selected 90 premature infants born in the neonatology department of our hospital over the past year, implemented the nursing measures combined with several nursing methods and reasonable and effective feeding methods according to the clinical manifestations of the premature infants, and observed the clinical effects. After a period of careful nursing, among these 90 premature infants, 2 infants died unfortunately, 4 infants abandoned the treatment, the remaining premature infants were discharged in good condition in all respects, could autonomously suck milk and reach the minimum daily suction volume, their weights increased to some extent, and their body temperatures could maintain normal under normal room temperature condition. According to the clinical experience, timely implementing the nursing measures combined with several nursing methods and reasonable and effective feeding methods to the premature infants can effectively reduce the morbidity and mortality of premature infants, and improve the survival rate and living quality of the premature infants, so that it is suitable for clinical promotion.

Abstract Background: To analyze the relative expression of PELI3 and its mechanistic involvement in the non-small cell lung cancer (NSCLC). Methods: PELI3 expression in NSCLC tissue samples was determined by the immunohistochemistry. The transcripts abundance of PELI3 was measured with real-time PCR. The protein intensity was analyzed by western blot. The overall survival in respect to PELI3 or miR-365a-5p expression was plotted by the Kaplan–Meier's analysis. Cell growth was determined by colony formation assay. Cell viability was measured by MTT assay. The migration and invasion were evaluated by wound healing and transwell assay respectively. The regulatory effect of miR-365a-5p on PELI3 was interrogated with luciferase reporter assay. The direct binding between miR-365a-5p and PELI3 was analyzed by pulldown assay. Results: PELI3 was aberrantly up-regulated in NSCLC both in vivo and in vitro. High level of PELI3 associated with poor prognosis. PELI3-deficiency significantly inhibited cell viability, colony formation, migration and invasion. We further identified that miR-365a-5p negatively regulated PELI3 in this disease. Ectopic expression of miR-365a-5p in both A549 and H1299 phenocopied PELI3-deficiency. Meanwhile, PELI3-silencing significantly abolished the pro-tumoral effect elicited by miR-365a-5p inhibition. Conclusion: Our results highlighted the importance of dysregulated miR-365a-5p-PELI3 signaling axis in NSCLC.